BIOCHEMICAL STUDIES ON PHOSPHATASE

Histochemical and biochemical studies on leukocyte alkaline phosphatase activity.

Biochemical Studies on Shock

During and following the production of shock by hemorrhage in the normal, suprareno-demedullated, and suprarenalectomized rat, the following significant changes in amino acid and carbohydrate metabolism have been observed. 1. In the intact, suprareno-demedullated, and suprarenalectomized rat there is a progressive rise in the whole blood and plasma amino acid nitrogen levels during and after a ...

Biochemical Studies on Inositol

In a previous publication (1, Paper V of this series) the purification and properties of the inositol-cleaving enzyme were described. It was shown that a highly purified enzyme was obtained from rat kidneys which cleaved inositol to D-ghCUI’OniC acid. The purified enzyme was shown to possess SH groups and 1 gram atom of iron per mole of enzyme. Both the SH groups and the iron are necessary for ...

Biochemical studies on regenerating liver.

Regenerating liver of the rat has long been recognized as a source of relatively abundant quantities of homogeneous rapidly growing tissue. Following the removal of the two largest lobes of the liver, the remaining ones, constituting about one-third of the total, enlarge to the size of the original liver in the span of a few days. The work of Higgins and Anderson (l), Brues et al. (2), and othe...

Biochemical Studies on Active Transport

The active transport process, so important in cell function, has been studied in the past with intact cells. Models which have arisen from this work all depend on: first, a specific protein to recognize the substrate; second, translocation of the substrate across the cell membrane; third, release of substrate within the cell and restoration of the system to its initial state. These steps are ad...